af960 rrid ab 355745 Search Results


af960 rrid ab 355745  (R&D Systems)
94
R&D Systems af960 rrid ab 355745
Af960 Rrid Ab 355745, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af960 rrid ab 355745/product/R&D Systems
Average 94 stars, based on 1 article reviews
af960 rrid ab 355745 - by Bioz Stars, 2026-02
94/100 stars
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biotinylated anti epcam antibody  (R&D Systems)
99
R&D Systems biotinylated anti epcam antibody
a Intra- and extracellular HLA-I protein expression in circulating tumor cells from two cohorts of 8 patients with prostate cancer. Dotted line represents median HLA-I expression in all patient matched white blood cells (WBCs). b Representative ×40 images of a CTC with low HLA-I expression and a WBC near the median expression from the total HLA-I group. Hoechst (Hst; blue; CD45/CD34/CD11b/CD27 (Excl; red); pan-cytokeratin (CK; white). Scale bars represent 10 µM. c Representative 10x images of HLA-I (yellow) undetectable and HLA-I detectable CTCs and a WBC (white arrow) in the single-cell aspirator microwells (Pt. 56) HLA-I (yellow), <t>EpCAM</t> (red), Excl. (white). Scale bars represent 10 µm. d Mean fluorescent intensity (MFI) of HLA-I in each CTC population and matched WBCs (where available). Statistical analysis was done by one-way ANOVA with the Kruskal–Wallis test, and Welch’s t -test for Pt 58. Approximately 10–15 CTCs were chosen to be aspirated from each group. e HLA-I methylation in 10 WBCs and 10 LNCaP cells purified by single-cell aspiration. f Relative LINE1 methylation in groups of ~10–15 CTCs purified by single-cell aspiration. g HLA-I methylation in HLA-I detectable and undetectable groups of ~10–15 CTCs purified by single-cell aspiration represented with a Methylation Index relative to methylation in LNCaP cells.
Biotinylated Anti Epcam Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated anti epcam antibody/product/R&D Systems
Average 99 stars, based on 1 article reviews
biotinylated anti epcam antibody - by Bioz Stars, 2026-02
99/100 stars
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Image Search Results


a Intra- and extracellular HLA-I protein expression in circulating tumor cells from two cohorts of 8 patients with prostate cancer. Dotted line represents median HLA-I expression in all patient matched white blood cells (WBCs). b Representative ×40 images of a CTC with low HLA-I expression and a WBC near the median expression from the total HLA-I group. Hoechst (Hst; blue; CD45/CD34/CD11b/CD27 (Excl; red); pan-cytokeratin (CK; white). Scale bars represent 10 µM. c Representative 10x images of HLA-I (yellow) undetectable and HLA-I detectable CTCs and a WBC (white arrow) in the single-cell aspirator microwells (Pt. 56) HLA-I (yellow), EpCAM (red), Excl. (white). Scale bars represent 10 µm. d Mean fluorescent intensity (MFI) of HLA-I in each CTC population and matched WBCs (where available). Statistical analysis was done by one-way ANOVA with the Kruskal–Wallis test, and Welch’s t -test for Pt 58. Approximately 10–15 CTCs were chosen to be aspirated from each group. e HLA-I methylation in 10 WBCs and 10 LNCaP cells purified by single-cell aspiration. f Relative LINE1 methylation in groups of ~10–15 CTCs purified by single-cell aspiration. g HLA-I methylation in HLA-I detectable and undetectable groups of ~10–15 CTCs purified by single-cell aspiration represented with a Methylation Index relative to methylation in LNCaP cells.

Journal: Communications Biology

Article Title: Reversible epigenetic alterations regulate class I HLA loss in prostate cancer

doi: 10.1038/s42003-022-03843-6

Figure Lengend Snippet: a Intra- and extracellular HLA-I protein expression in circulating tumor cells from two cohorts of 8 patients with prostate cancer. Dotted line represents median HLA-I expression in all patient matched white blood cells (WBCs). b Representative ×40 images of a CTC with low HLA-I expression and a WBC near the median expression from the total HLA-I group. Hoechst (Hst; blue; CD45/CD34/CD11b/CD27 (Excl; red); pan-cytokeratin (CK; white). Scale bars represent 10 µM. c Representative 10x images of HLA-I (yellow) undetectable and HLA-I detectable CTCs and a WBC (white arrow) in the single-cell aspirator microwells (Pt. 56) HLA-I (yellow), EpCAM (red), Excl. (white). Scale bars represent 10 µm. d Mean fluorescent intensity (MFI) of HLA-I in each CTC population and matched WBCs (where available). Statistical analysis was done by one-way ANOVA with the Kruskal–Wallis test, and Welch’s t -test for Pt 58. Approximately 10–15 CTCs were chosen to be aspirated from each group. e HLA-I methylation in 10 WBCs and 10 LNCaP cells purified by single-cell aspiration. f Relative LINE1 methylation in groups of ~10–15 CTCs purified by single-cell aspiration. g HLA-I methylation in HLA-I detectable and undetectable groups of ~10–15 CTCs purified by single-cell aspiration represented with a Methylation Index relative to methylation in LNCaP cells.

Article Snippet: Fixed cells were incubated with paramagnetic particles (PMPs) (Dynabeads® FlowCompTM Flexi kit, Life Technologies, Cat# 11061D) coated with biotinylated anti-EpCAM antibody (R&D Systems, Cat# AF960, RRID: AB_355745).

Techniques: Expressing, Methylation, Purification